Abhay Deep Johnson1, Ajay Singh2*
1Research scholar, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur,
(U.P.) India
2Professor, Department of Zoology, D.D.U.
Gorakhpur University, Gorakhpur, (U.P.) India
*Address for Correspondence: Dr. Ajay
Singh, Professor,
Department of Zoology, D.D.U.
Gorakhpur University, Gorakhpur, (U.P.) India
ABSTRACT- Mosquito-borne diseases have intruded the
globe since immemorial time. The present scenario for commanding the mosquitoes
is aimed at application of target and stage-specific, cost-effective and
biodegradable phytoproducts. Plant extracts are safer for non-target organisms
including man. Plant based formulations would be more feasible environmental
products with proven potential as insecticide. Therefore, in the present study
of larvicidal activity of biologically active compound Apigenin extracted from
leaf of Jatropha gossypifolia against
the filarial vector, Culex quinquefasciatus was studied. Standard WHO protocols with minor modifications
was adopted for the larvicidal bioassay. The active compound Apigenin extracted
through ethyl alcohol solvent from the leaf of Jatropha gossypifolia plant of family Euphorbiaceae was
administered for 24h or 96h to the larvae of Culex quinquefasciatus. Exposure of larvae over 24h to sub-lethal
doses (40% and 80% of LC50) of apigenin, significantly (P<0.05)
altered the level of total protein, total free amino acid, glycogen and
activity of enzymes acetyl cholinesterase, acid and alkaline phosphatase
activity in whole body tissue of Culex
quinquefasciatus larvae. The alterations in all these biochemical
parameters were significantly (P<0.05) time and dose dependent.
Key-words- Jatropha
gossypifolia,
Euphorbiaceae, Culex quinquefasciatus,
biochemical effects, Wuchereria bancrofti
