Anti-cancer Activity of Leaf Extract Preparation from Ipomoea sepiaria against PC-3 Cell Line

Sudhakar Meesala^1*, Satyanarayana Rentala², DSVGK Kaladhar³

¹Research scholar, Department of Biotechnology, Jawaharlal Nehru Technological University Hyderabad, India

²Associate Professor, Department of Biotechnology, GITAM University, Visakhapatnam, India

³Associate Professor, Dept. of Microbiology and Bioinformatics, Bilaspur University, Bilaspur, India

 ^*Address for Correspondence: Mr. Sudhakar Meesala, Research scholar, Department of Biotechnology, JNTUH, Hyderabad, India

  

ABSTRACT- Researches on PC-3 human prostate cancer cell lines control are needed in the present decades. The anticancer activity of aqueous extract of Ipomoea sepiaria was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay using PC-3 cell line. The present experimentation was showed that aqueous extract of Ipomoea sepiaria, when subjected to different concentrations on PC-3 cells showed IC50 cell inhibition at about 5µM for 48 hours and about 2µM for 72 hours. PTEN interacts with other expression proteins like Jun proto-oncogene, V-akt murine thymoma viral oncogene homolog 1 and 2, Tumor protein p53, Phosphatidylinositol-4,5-bisphosphate 3-kinase, etc. The present experiment shown that the leaf extract may involve in protein suppressor mechanism for PTEN in controlling of prostate cancer.

Key-words- PC-3, Ipomoea sepiaria, Anticancer activity, MTT assay, Prostate cancer

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